By Darsa P Siantar, Mary W Trucksess, Peter M Scott, Eliot M Herman
content material: Mycotoxins : toxicology and keep watch over --
Mycotoxins : approach to research --
meals allergens : allergic capability and keep watch over --
nutrition asthma : approach to analysis.
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Additional info for Food Contaminants. Mycotoxins and Food Allergens
Liver nuclear DNA, enriched in ribosomal DNA (rDNA) by one round of cesium salt density gradient centrifugation, was treated under buffered alkaline conditions to convert unstable AFB N -guanine adducts to stable AFBi-FAPY derivatives. rDNA contained 4- to 5-fold more AFBi residues than nuclear DNA, indicating that rDNA is preferentially accessible to carcinogen modification in vivo. While AFBi forms adducts with DNA principally at guanine residues, the guanine enrichment of rDNA was insufficient to explain the magnitude of observed preferential AFBi modification of rDNA.
The broad specificity A F B antibody might be of considerable value in the detection of AFBi macromolecular adducts and related metabolites in epidemiological investigations or in the diagnosis of aflatoxicosis. Garner et al. (91) used immunoassay procedures to detect exposure to AFBi and benzo[a]pyrene in animals and man at the DNA level. Hsieh et al. (9) developed monoclonal antibodies after fusion of mouse myeloma cells (Sp ) with spleen cells and developed an immunological detection of AFBpDNA adducts formed in vivo.
ACS Symposium Series; American Chemical Society: Washington, DC, 2008. 38 Control There are several options to diminish or control the problem of the presence of adducts in the DNA of an organism that can cause a mutation and maybe a carcinogenic process. These possibilities go from natural repair rates, implicated enzymes, chemicals, and natural products. ch002 Natural Repair Rates and Adduct Removal Natural repair rates in the hamster and rat were constant over time with removal of the 8,9-dihydro-8-(N -guanyl)-9-hydroxy AFBi accounting for the majority of adduct disappearance.