By Ana M. Garcia-Campana
Discusses static ideas and flowing movement measurements of chemiluminscence utilized to chemical research, with stories of advances in immunoassay thoughts and makes use of of enzyme reactions. comprises attention of a variety of purposes in analytical study, biotechnology and chemical quality controls.
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Extra info for Chemiluminescence in Analytical Chemistry
JD Ingle, SR Crouch. Spectrochemical Analysis. Englewood Cliffs, NJ: PrenticeHall, 1988, pp 478–485. 219. JL Burguera, M Burguera, A Townshend. Acta Cient Venezolana 32:115–122, 1981. 220. LJ Kricka. GHG Thorpe. Analyst 108:1274–1296, 1983. 221. JL Bernal, V Cerda´. Quı´m Anal 4:205–235, 1983. 222. ML Grayeski. Anal Chem 59:1243 A-1256 A, 1987. 223. M DeLuca, LJ Kricka, WD McElroy. Trends Anal Chem 1:225–228, 1982. 224. A Fontijn, ed. Gas-Phase Chemiluminescence and Chemi-Ionization. New York: Elsevier, 1985.
Outstanding research—among others—on the use of aryl oxalates for CL detection in high-performance liquid chromatography (HPLC) was introduced in the 1980s by Imai’s group from Tokyo University. They determined fluorescers 30 Garcı´a-Campan˜a et al. or fluorophore-labeled compounds by HPLC and CL detection , using TCPO in ethyl acetate, and hydrogen peroxide in acetone, mixed with the column eluate using separate pumps and a suitable mixing vessel. Dansyl amino acids (alanine, glutamic acid, methionine, and norleucine) were separated on a µ-Bondapak C18 column and fmol limits could be reached.
16 Garcı´a-Campan˜a et al. Some of the investigations carried out in the first half of the twentieth century were related to CL associated with thermal decomposition of aromatic cyclic peroxides [75, 76] and the extremely low-level ultraviolet emission produced in different reaction systems such as neutralization and redox reactions involving oxidants (permanganate, halogens, and chromic acid in combination with oxalates, glucose, or bisulfite) . In this period some papers appeared in which the bright luminescence emitted when alkali metals were exposed to oxygen was reported.