By G. Subramanian, Ganapathy Subramanian
Fast advancements in biotechnology create a requirement for useful, updated studies written by means of and for specialists in undefined. This compact guide presents all suitable updated details on very important bioseparation and bioprocessing options which are actively utilized within the biotechnology industries. The instruction manual offers an applications-orientated review on - case stories and normal options for quality controls and characterization - certain guidance on constructing fiscal and technically possible bioseparation schemes - suggestions and strategies for intracellular bioproduct unencumber - chromatographic and membrane downstream techniques utilized in biotechnology - purposes of recent non-invasive equipment corresponding to neural networks for online estimation and keep watch over of fermentation variables on an commercial scale - a realistic, commercially-relevant consultant to biosafety and plenty of extra elements that are indispensible for current and destiny commercial luck.
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Extra info for Bioseparation and Bioprocessing, 2 Vol set
2 Influence of Number of Theoretical Plates The fact that SMB is less sensitive with respect to the number of theoretical plates than batch chromatography is probably due to the fact that SMB involves a counter-current contact between the liquid and the solid phases. This difference in behavior has been recognized by Nicoud and Bailly  and quantified by Tondeur and Bailly . In order to explain this behavior, let us consider the curve given in Fig. 1-17 in which a dimensionless loadability is given versus the number of plates equivalent to the system considered.
3 7 I T_ ~ 0 - I 1 m m m Number of plates Number of plates Fig. 1-18. Comparison of eluent consumption for batch chromatography and for SMB: influence of the selectivity factor (Langmuir behavior) and of the number of plates. 5.
Another possibility consists of using anion exchange resins in the bisulfite form, the glucose being retarded by complexing with the other oligosaccharides being eluted first. This option is not used at production scale because of the lower stability of anion-exchange resins. The Sarex process has been reported  to be used to separate continuously a 500 g 1-' inverted carbohydrate syrup containing 42% fructose giving 90-94% pure fructose at recovery of over 90%. The glucose-rich fraction is about 80% and both product concentration were about 200 g/l.