By A H Rebar; et al
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Additional resources for A Guide to Hematology in Dogs and Cats
Whenever increased polychromasia is seen on routine blood films, reticulocyte counts are warranted. Reticulocyte counts of greater than 80,000/µl indicate regeneration. Regenerative anemias are either the result of blood loss or hemolysis. Regenerative Anemia: Blood Loss / Hemorrhage History, clinical signs, and physical examination often point to the source of blood loss. In general, blood loss anemias are less responsive than hemolytic anemia. The main reason is that when blood loss occurs, iron is lost from the body and iron is needed for hemoglobin synthesis.
Cells touching bottom and right inner lines are not counted. , Ames, IA, the Iowa State University Press, 1978 Figure 3-11 Unopette® diluting pipette and reservoir for counting WBCs and platelets in a hemocytometer. 20 TNM GuideToHema:03058 TNM text_cp 6/28/07 3:49 PM Page 21 3 LABORATORY METHODS IN HEMATOLOGY ® Platelet count – Can be performed using hemocytometer and ammonium oxalate diluent but it is difficult to achieve accuracy even among skilled medical technologists. – Platelet count can be estimated from the blood smear.
Followed by monocytes and lymphocytes the granulocytes are the most dense of the white cells and come to rest immediately on top of the erythrocytes. ® Uses special acridine orange-coated tubes that include a cylindrical float with the same density as the buffy coat. When a tube is centrifuged, the float effectively reduces the diameter of the tube in the region of the buffy coat, causing it to spread along a greater length of the tube, enabling resolution of the various layers. ® The acridine orange stains nucleoproteins and other cellular components, which fluoresce when exposed to ultraviolet light.